Part:BBa_K769021:Experience

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Applications of BBa_K769021

Evaluation

Transformed E coli. TOP10 was precultured into LB medium and incubated at 37℃. After incubating for 12 hour, precultured solution was diluted with LB medium to the point that OD660 became 0.6, then applied into 96well microtiter plate. After adding arabinose(0.25% f.c.), the plate was shaken at 20 ℃ to express the lux proteins. The spectrum of the light and intensity was measured by plate reader once in an hour.

Result

We could not observe the yellow light from lux+YFP construct (Figure.c). We assumed that the yellow shift didn’t occur due to difference of luminous bacteria strain (YFP is from Vivrio fischeri.).

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